Read e-book Gel Chromatography: Gel Filtration · Gel Permeation · Molecular Sieves: A Laboratory Handbook

Free download. Book file PDF easily for everyone and every device. You can download and read online Gel Chromatography: Gel Filtration · Gel Permeation · Molecular Sieves: A Laboratory Handbook file PDF Book only if you are registered here. And also you can download or read online all Book PDF file that related with Gel Chromatography: Gel Filtration · Gel Permeation · Molecular Sieves: A Laboratory Handbook book. Happy reading Gel Chromatography: Gel Filtration · Gel Permeation · Molecular Sieves: A Laboratory Handbook Bookeveryone. Download file Free Book PDF Gel Chromatography: Gel Filtration · Gel Permeation · Molecular Sieves: A Laboratory Handbook at Complete PDF Library. This Book have some digital formats such us :paperbook, ebook, kindle, epub, fb2 and another formats. Here is The CompletePDF Book Library. It's free to register here to get Book file PDF Gel Chromatography: Gel Filtration · Gel Permeation · Molecular Sieves: A Laboratory Handbook Pocket Guide.

The separated components are visualized as a plot of the volume of the mobile phase eluted through the column versus the detector signal. This plot, called a chromatogram, shows the location of the individual peaks and the quality of resolution of these peaks. In Figure 1, there are two well-separated peaks corresponding to the two components A and B in the sample. Component A red requires solvent of volume V e A to elute at its maximum concentration. Component B green has near complete access to the pores of the matrix.

Therefore, its flow through the column is retarded. It elutes just before one column volume worth of the solvent can pass through the column i. The location of the peaks, their heights, and widths convey a great deal of information about the sample components as well as the column. Importantly, you can use peak locations to determine the molecular weights of the sample components.

The distribution of an analyte between the stationary and mobile phases depends on its size. It is described by a parameter called its equilibrium distribution coefficient K d. K d is the ratio of the concentrations of the component in the stationary and the mobile phases. For component B, K d can be written as. So, K d depends on particle size; therefore, it is essential to determine K d to estimate particle size. It is difficult to determine the concentrations of an analyte in the stationary and mobile phases.

Therefore, you express K d in terms of the easily measurable parameters, V e and V o. We know that the flow of component B through the column is retarded and it elutes at volume V e B. V T is the total solvent accessible volume, a parameter that is difficult to measure. Recent citations: Sneha Munshi et al. Related articles Based on techniques. MacKinnon , , Springer Protocols less.

References Tanford C Protein denaturation. Adv Protein Chem — Uversky VN Use of fast protein size-exclusion liquid chromatography to study the unfolding of proteins which denature through the molten globule. Biochemistry — Uversky VN Gel-permeation chromatography as a unique instrument for quantitative and qualitative analysis of protein denaturation and unfolding. A polypeptide chain at the folding-misfolding-nonfolding cross-roads: which way to go? Nature — Porath J Molecular sieving and adsorption. Nature — Andrews P The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

Equations for transport of associating systems. Interpretation of calibration curves in terms of gel-pore-size distribution. Sequestration of crystalline and endogenous cobalamin by R binders down to the distal ileum in exocrine pancreatic dysfunction. Steven D. Cadmium causes vesicle leakage under conditions which favor reconstitution of tissue factor-vesicle complexes.

The Journal of Membrane Biology , 75 2 , Nicholas Catsimpoolas. Chapter 11 Proteins. Chapter 3 Theory of chromatography. Porro, S. Fabbiani, I. Marsili, S. Viti, M. Immunoelectrophoretic characterization of the molecular weight polydispersion of polysaccharides in multivalent bacterial capsular polysaccharide vaccines.

Journal of Biological Standardization , 11 1 , Robert P. Bywater, N. Chapter 8 Gel chromatography. Comparison of some physicochemical and kinetic properties of S-Adenosylhomocysteine hydrolase from bovine liver, bovine adrenal cortex and mouse liver. European Journal of Biochemistry , , Rakonczay, G. Vincendon, J-P. Journal of Neurochemistry , 37 3 , Gorbunov, E.

Zhulina, A. Theory of adsorption of macromolecules in cylindrical pores and at surfaces of cylindrical shape. Polymer , 23 8 , Richard G. Properdin is a trimer. Molecular Immunology , 19 4 , Phenylalanine 4-monooxygenase from bovine and rat liver: Some physical and chemical properties. Neurochemical Research , 7 4 , Alexander Currie, John A. Physicochemical properties of the cytoplasmic glucocort1coid receptor complex in HeLa S3 cells. Journal of Steroid Biochemistry , 16 3 , Hamilton, P. Introduction to high performance liquid chromatography. Sandra L. Lee, Melvin J. Purification, composition, and31P NMR spectroscopic properties of a noncollagenous phosphoprotein isolated from chicken bone matrix.

Calcified Tissue International , 33 1 , Lawrence G. Hunsicker, Thomas P. Shearer, Sandra J. Acute reversible proteinuria induced by infusion of the polycation hexadimethrine. Kidney International , 20 1 , Heterologous enzyme-enzyme complex between d-fructose-1,6-bisphosphate aldolase and triosephosphate isomerase from Ceratitis capitata.

In vivo evidence that intrinsic factor-cobalamin complex traverses the human intestine intact. Pier Giorgio Righetti, Brian C. Brost, Robert S. On the limiting pore size of hydrophilic gels for electrophoresis and isoelectric focussing. Journal of Biochemical and Biophysical Methods , 4 , Characterization of an acidic glycoprotein secreted by principal cells of the rat epididymis.

Jack L. Leonard, Isadore N. Don R. Yeltman, Goeh Jung, Kermit L. Shigenoki Kuga. Pore size distribution analysis of gel substances by size exclusion chromatography. Journal of Chromatography A , 3 , Purification and characterization of rabbit haptocorrin. Annette Vigny, Jean-Pierre Henry. Journal of Neurochemistry , 36 2 , Rakonczay, J. Mallol, H.

E. Gross (Author of א איד איז קיינמאל נישט אליין )

Schenk, G. Vincendon, J. Purification and properties of the membrane-bound acetylcholinesterase from adult rat brain. Francisco G. Triosephosphate isomerase from the insect Ceratitis capitata molecular and enzymic properties. Chromatography Theory. Ilkka Kouvonen. Solubilization of the pig ileal intrinsic factor receptor with papain treatment and studies on the solubilized receptor.

Immunochemical and ultrastructural study of multiple myeloma with a heavy chain protein in the serum.. Journal of Clinical Pathology , 33 10 , In Cheol Kim. Isolation and properties of somatic and testicular cytochromes c from rat tissues. Use of gel chromatography for determination of size and molecular weight of proteins: Further caution. Ellen Sue Lyon, William B. The identity of alcohol sulfotransferases with hydroxysteroid sulfotransferases. International Journal of Peptide and Protein Research , 16 1 , Bragado, J. Albar, C. Juarez, F. Molecular Immunology , 17 3 , The biological and physico-chemical characteristics of meningococcus polysaccharide group C prepared by two different methods of purification.

Journal of Biological Standardization , 8 1 , Theoretical considerations of molecular sieve effects. Journal of Chromatography A , 2 , ROY L. Gel Permeation Chromatography. John H. Knox, Forbes McLennan. Band dispersion in high-performance exclusion chromatography. Medgyesi, G. Gergely, J. Activation of the classical complement pathway by homogeneous anti-SIII antibody bound to bivalent or trivalent oligosaccharide antigens.

Molecular Immunology , 16 11 , Kasuga, T. Tsushima, Y. Akanuma, K. Solubilization of insulin binding and degrading activity from guinea pig kidneys. Diabetologia , 17 5 , Michetti, V. Singh, B. Evidence for an interaction between fructose 1,6-bisphosphatase and fructose 1,6-bisphosphate aldolase. Nibaldo C. Inestrosa, Miguel Bronfman, Federico Leighton.

Properties of fatty acyl-CoA oxidase from rat liver, a peroxisomal flavoprotein. Life Sciences , 25 13 , Structure of Pyridine Nucleotide Transhydrogenase from Azotobacter vinelandii. European Journal of Biochemistry , 98 2 , Journal of Chromatography A , 1 , European Journal of Biochemistry , 97 1 , Fernandez-Sousa, J.

Gavilanes, J. Paredes, A. A method for the isolation of triose phosphate isomerase, l-Glycerol 3-phosphate dehydrogenase, d-Fructose 1,6-bisphosphate aldolase and cytochrome from a single extract of Ceratitis capitata flies. Insect Biochemistry , 9 2 , Chapter 9 Silica as packing in size-exclusion chromatography. William A. Coty, William T. Schrader, Bert W. Purification and characterization of the chick oviduct progesterone receptor a subunit.

Journal of Steroid Biochemistry , 10 1 , Tim Hunt. Chapter 4 Molecular Size and Shape in Electrophoresis. Schwenke, W. Linow, B. Raab, M. On seed proteins Part Plant Microbody Proteins. European Journal of Biochemistry , 92 1 , Hasko Paradies, Wolfgang Vettermann. Small-angle X-ray scattering of phosphofructokinase from Dunaliella salina. Bernard Colas. Potter, Patrick H. Stafford, Susan Taylor. Regulatory subunit of cyclic AMP-dependent protein kinase I from porcine skeletal muscle: Purification and proteolysis.

Characterization of the physicochemical and catalytic properties of human heart NADP-dependent isocitrate dehydrogenase. Gavilanes, Jose G. Helmut G. Rennke, Yogendra Patel, Manjeri A. Glomerular filtration of proteins: Clearance of anionic, neutral, and cationic horseradish peroxidase in the rat. Kidney International , 13 4 , Garth E. Purification and properties of polynucleotide kinase of calf thymus. Van Kreveld, N. Van den Hoed. Mass transfer phenomena in gel permeation chromatography. International Journal of Peptide and Protein Research , 11 2 , Thomas W.

Traut, Mary Ellen Jones. Advances in Enzyme Regulation , 16 , Dermot M. Malone, John L. Hindered diffusion of particles through small pores. Chemical Engineering Science , 33 11 , Koichi Saitoh, Nobuo Suzuki. The Partition Concept in Gel Chromatography. Bulletin of the Chemical Society of Japan , 51 1 , Isolation of vitamin Bbinding proteins by combined immuno and affinity chromatography. European Journal of Biochemistry , 80 2 , Stephen A. Brown, Dexter French. Specific adsorption of starch oligosaccharides in the gel phase of starch granules.

Carbohydrate Research , 59 1 , Olesen, D. Bucher, J. Purification and characterization of rabbit transcobalamin II. Yeltman, Ben G. Purification and characterization of aldolase from human erythrocytes. Isolation and identification of a trypsin-resistant fragment of human serum albumin with bilirubin- and drug-binding properties. J Hewitt, B. A Notton, G. J Rucklidge.

Formation of nitrate reductase by recombination of apoprotein fractions from molybdenum-deficient plants with a molybdenum-containing complex. Journal of the Less Common Metals , 54 2 , Morelli, U. Benatti, L. Radin, N. Wrigley, A. De Flora. FEBS Letters , 80 1 , Appaji RAO. Studies on Nucleotidases in Plants. European Journal of Biochemistry , 78 1 , Marcoullis, R. Identification and characterization of intrinsic factor and cobalophilin from pig ileal and pyloric mucosa. Porter, Barbara E. Partial purification and characterization of citrate synthase from Dictyostelium discoideum.

The isolation of microquantities of myosin from Amoeba proteus and Chaos carolinensis. Analytical Biochemistry , 78 2 , Dolara, F. Franconi, N. The effect of phalloidin on natural and U. V denatured bovine serum albumin. Pharmacological Research Communications , 9 4 , Municio, M. Lizarbe, E. Fatty acid synthetase complex from the insect Ceratitis capitata. Gavilanes, A.

Municio, A.

L-glycerolphosphate dehydrogenase from the insect Ceratitis capitata Purification, physicochemical and enzymic properties. Haller, A.

About This Item

Basedow, B. General permeation chromatography equation and its application to taylor-made controlled pore glass columns. Applications of gel permeation chromatography in the paint industry. Progress in Organic Coatings , 5 2 , Solubilised intrinsic factor receptor from pig ileum and its characteristics. Unsaturated and cobalamin saturated transcobalamin I and II in normal human plasma. Scandinavian Journal of Clinical and Laboratory Investigation , 37 8 , Hippe, F. Cobalamin binding proteins in stomach and serum from various animal species data for B12 binding capacities and molecular sizes of the binding proteins.

Wiegel, H. Archives of Microbiology , 3 , Jose M. Lysozyme from the Insect Ceratitis capitata Eggs. European Journal of Biochemistry , 72 1 , Yamamoto, Y. Solvent effects in gel chromatography: the relationship between the distribution coefficients of metal acetylacetonates and solubility parameters of solvents. Analytica Chimica Acta , 87 2 , Barry H. Ginsberg, C. Insulin-induced dissociation of its receptor into subunits: Possible molecular concomitant of negative cooperativity.

Biochemical and Biophysical Research Communications , 73 4 , Human Coagulation Factor IX. Isolation and Characterization. European Journal of Biochemistry , 71 1 , Physico-chemical characterization of the spectrin tetramer from bovine erythrocyte membranes. Chakwan Siew, Richard A. Deitrich, V. Gene Erwin. Localization and characteristics of rat liver mitochondrial aldehyde dehydrogenases.

Development, Growth and Differentiation , 18 3 , George R. Duncombe, Frank E. Molecular and catalytic properties of the acetoacetyl-coenzyme A thiolase of Escherichia coli. Turco, Edward C. The molecular and catalytic properties of galactosyltransferase from fetal calf serum. Donald N. Neff, Aleck Bernstein. Purification and physical characterization of colicin 0 R Anaya-Galindo, S.

J Shattil, J. C Shelburne, R. W Colman. Soluble fibrin complexes in coronary disease. Thrombosis Research , 9 2 , Geometry of the Protein S4 from Escherichia coli Ribosomes. European Journal of Biochemistry , 67 1 , Angelina C. Carvalho, Robert S. Lees, Raymond A. Vaillancourt, Rae B. Cabral, Robert M. Weinberg, Robert W. Intravascular coagulation in hyperlipidemia. Thrombosis Research , 8 6 , Yoshitsugu Naito, Keizo Tsushima.

Gel Chromatography

Susan J. Cavalieri, David A. Goldthwait, Kenneth E. The isolation of a dimer of gene 8 protein of bacteriophage fd. Journal of Molecular Biology , 4 , John G. Duman, Arthur L. Isolation, characterization, and physical properties of protein antifreezes from the winter flounder, Pseudopleuronectes americanus. Joseph G. Kunkel, M. Selectivity of yolk protein uptake: Comparison of vitellogenins of two insects. Journal of Insect Physiology , 22 6 , Samuel Zigler, J. Knut Wallevik.

SS-interchanged and oxidized isomers of bovine serum albumin separated by isoelectric focusing. Margaret L. Fonda, Sidney B. The interaction of pyridoxal phosphate with aspartate apoaminotransferase. Brewer, Lars G. David Rodbard. Correlations with Extrathermodynamic Parameters. Robert M. Reconstitution of potato virus X in vitro. Virology , 68 2 , David J. Glutathione Reductase from Human Erythrocytes. European Journal of Biochemistry , 60 2 , Stephen J. Sramek, Frank E.

Purification and properties of Escherichia coli coenzyme A-transferase. Bourbonnais, R. Guevin, E. Purification and Partial Characterization. Vox Sanguinis , 29 4 , Venkateswara Rao, Elijah Adams. Observations on the gel chromatography of collagen-like peptides and use of a simple calibration method for molecular weight determination. Analytical Biochemistry , 68 1 , European Journal of Biochemistry , 55 3 , Belenkii, L. Vilenchik, V.

Nesterov, V. Kolegov, S. Peculiarities in gel permeation chromatography of flexible-chain polymers on macroporous swelling sorbents. Jonathan Craine, Alan Peterkofsky. Evidence that arginyl-adenylate is not an intermediate in the arginyl-tRNA synthetase reaction. Balakrishnan, S. Ravindranath, N. Appaji Rao. Studies on nucleotidases in plants. International Journal of Peptide and Protein Research , 7 3 , Donald J.

Roufa, R. Moses, Susan J. The DNA polymerases of Chinese hamster cells. Stephen Moore, Duncan S. Pepper, John D. Platelet antiheparin activity. Tong Hyub Joh, Donald J. Different forms of tyrosine hydroxylase in central dopaminergic and noradrenergic neurons and sympathetic ganglia. Brain Research , 85 1 , Incorporation and metabolic conversion of cyanocobalamin by ehrlich ascites carcinoma cells in vitro and in vivo. GARY K. Molecular Sieve Methods of Analysis.

Chapter 5 Gel permeation chromatography.

  • The Reaction (Animorphs, Book 12);
  • The Nature of Life.
  • The Working Principles of Gel Permeation Chromatography;
  • CNA - 一种大型凝胶层析柱纯化有机大分子物质的工艺 - Google Patents!
  • The Advanced Econometrics of Tourism Demand (Routledge Advances in Tourism).

Merry R Sherman. Chapter 12 Practice of gel chromatography. Vilenchik, B. Belen'kii, V. Gel permeation chromatography of flexible polymers on macroporous, swollen absorbents. Polymer Science U. Chapter 51 Inorganic, coordination and organometallic compounds. The effect of anions on the interaction of pyridoxal phosphate with glutamate apodecarboxylase.

Archives of Biochemistry and Biophysics , , Scandinavian Journal of Clinical and Laboratory Investigation , 35 2 , Wayne W.